Optogenetics
AAV2/9-hEF1a-fDIO-ChrimsonR-EGFP-ER2-WPRE-pA
AAV2/9
ChrimsonR
hEF1a
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Delivery Information
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Product Details
Promoter Details
Ef1α, a strong promoter, human targeted elongation factor 1α (EF1A) gene, can be stably expressed in most types of cells, especially cells where the CMV promoter is silenced, such as stem cells, 1178bp.
hE F1 a promoter, cloned from the promoter region of human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), is 1.3kb in length, constitutively expressed, and has no cell tissue specificity.
Functional Gene Details
ChrimsonR: A new type of light-controlled ion channel that can respond to red light around 600nm was discovered through high-throughput sequencing in the Boyden laboratory. A point mutation of K176R was made on the basis of Chrimson (CnChR1), which speeds up the channel closing speed, making it possible to effectively respond to light signals at least 20Hz and below. When used with Chronos, it is possible to control two different neurons with two fluorophores. The excitation light wavelength is 590nm.
Fluorescent Tag
green fluorescent green
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
constitutive expression
