Optogenetics
AAV2/9-CaMKIIa-hChR2(E123A)-mCherry
AAV2/9
ChETA
mCaMKIIa(1.3k)
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.
Functional Gene Details
ChETA: There is a point mutation of E123T, which accelerates the closing speed of the ion channel after light withdrawal, making the channel more sensitive to changes in light signals. There is also a mutation of hChR2 (H134R) to increase the inward current. Experiments have shown that ChETA can excite action potentials at a frequency of 200Hz in some neurons, and it is a commonly used high-frequency excitation tool. Compared with ChR2, its maximum excitation wavelength is red-shifted by about 20nm, but it can generally be excited with blue light of about 470nm. The excitation light wavelength is 490nm, suitable for high-frequency light activation.
Fluorescent Tag
red fluorescent red
Fluorescent Protein Details
dsRed, red fluorescent protein, excitation wavelength 557nm, emission wavelength 579nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
specific expression
