Excitatory Optogenetics
AAV2/9-CAG-FLEX-oChIEF-tdTomato-WPRE-pA
AAV2/2Retro, AAV2/9
oChIEF
CAG-FLEX CAG
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Product Details
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Promoter Details
CAG, strong promoter, an artificially constructed combined promoter, composed of cytomegalovirus early enhancer and chicken β-actin promoter, 1731bp.
The C AG promoter is a combination of the CMV promoter (C), the chicken β-actin intron (A) and the rabbit β-globin intron region (G). It is a strong constitutive promoter with no cell tissue specificity.
Functional Gene Details
OChIEF: ChIEF is a fusion of two proteins, ChR1 and ChR2, with Ile mutated to Val at position 170. The characteristic of this protein is that under long-term illumination, the ratio of plateau current to peak current is large, which means that the channel is less inactivated under illumination and is suitable for long-term stimulation. The mutation of Ile accelerates the closing speed of the channel, making ChIEF suitable for high-frequency stimulation. Karl's laboratory has compared the high-frequency stimulation of ChIEF and hChR2(E123A) in pv (small serum protein) neurons, and the effects are equivalent. OChIEF is a codon-optimized version. The excitation light wavelength is 473nm, suitable for high-frequency light activation.
Fluorescent Tag
Orange fluorescent Orange
Fluorescent Protein Details
mOrange: Orange fluorescent protein, excitation wavelength 548nm, emission wavelength 562nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
constitutive expression
