Optogenetics
AAV2/8-gfaABC1D-hChR2(H134R)-mCherry-ER2-WPRE-pA
AAV2/8, AAV2/5
hChR2 hChR2(H134R)
gfaABC1D
Store at -80°C
Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
GfaABC1D, human small version of astrocyte-specific promoter, 992 bp.
The gfaABC1D promoter is a simplified version of the GFAP promoter with similar specificity and function, but is only 1kb in length.
Functional Gene Details
hChR2(C128S/D156A/T159C): The excitation wavelength is 470nm, no need to implant optical fiber, and light stimulation is applied from outside the skull.
hChR2 (H134R): It is the most common form of ChR2. h refers to the coding sequence of the gene that has been optimized for the human codon table. H134R refers to the mutation of histidine at position 134 to arginine. This mutation inhibits the binding of H+ by ChR2 and increases the passage ability of Na+, thereby increasing the overall conductivity of the molecule and thereby increasing the inward current excited by light. Generally, blue light around 470nm is used for excitation.
Fluorescent Tag
red fluorescent red
Fluorescent Protein Details
dsRed, red fluorescent protein, excitation wavelength 557nm, emission wavelength 579nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
specific expression
