Inhibitory Optogenetics
AAV2/5-mCaMKIIa-eNpHR 3.0-tdTomato-WPRE-pA
AAV2/5
NpHR
mCaMKIIa(1.3k)
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Delivery Information
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Product Details
Promoter Details
CaMKIIa, pyramidal neuron/excitatory neuron promoter, 1293bp.
m C aMKIIa promoter, cloned from the promoter region of mouse calmodulin-dependent protein kinase IIa, is 1.3kb in length and is specifically expressed in excitatory neuronal cells.
Functional Gene Details
eNpHR3.0: NpHR is a light-driven chloride ion pump belonging to the Halorhodopsin family. The common version is NpHR3.0, which adds an endoplasmic reticulum anterograde transport signal and a Golgi transport signal to the cell membrane based on NpHR, improving its expression on the cell membrane. It is the most commonly used photoinhibition tool. NpHR is usually driven by yellow light around 570nm and can be used simultaneously with hChR2 (H134R).
Fluorescent Tag
Orange fluorescent Orange
Fluorescent Protein Details
mOrange: Orange fluorescent protein, excitation wavelength 548nm, emission wavelength 562nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
specific expression
