Optogenetics
AAV2/2-hSyn-hChR2(H134R)-tdTomato-ER2-WPRE-pA
AAV2/2, AAV2/8, AAV2/9
hChR2(H134R) hChR2
hSyn
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Delivery Information
Usually Ships in 7 Business Days；
Product Details
Promoter Details
hSyn, human synapsin promoter, mature neuron-specific promoter, 448bp.
The hSyn promoter, also known as the hSyn1 promoter, is cloned from the promoter region of human synapsin protein 1 (Synapsin1). It is about 0.5kb in length and is specifically expressed in neurons.
Functional Gene Details
hChR2 (H134R): It is the most common form of ChR2. h refers to the coding sequence of the gene that has been optimized for the human codon table. H134R refers to the mutation of histidine at position 134 to arginine. This mutation inhibits the binding of H+ by ChR2 and increases the passage ability of Na+, thereby increasing the overall conductivity of the molecule and thereby increasing the inward current excited by light. Generally, blue light around 470nm is used for excitation.
hChR2(C128S/D156A/T159C): The excitation wavelength is 470nm, no need to implant optical fiber, and light stimulation is applied from outside the skull.
Fluorescent Tag
green fluorescent green
Orange fluorescent Orange
Fluorescent Protein Details
mOrange: Orange fluorescent protein, excitation wavelength 548nm, emission wavelength 562nm.
Virus Type
Adeno-associated virus vector Adeno-associated Virus
Expression and Regulation Mode
specific expression
