RACE Primer Design Service

Product Introduction
Our RACE Primer Design Service provides customized primer design for Rapid Amplification of cDNA Ends (RACE) experiments, supporting both 5' RACE and 3' RACE applications. The service is designed to help researchers identify unknown transcript ends, obtain full-length cDNA sequences, verify transcript boundaries, and support gene cloning, transcript annotation, and expression studies.

We can design 5' gene-specific primers (5' GSP) or 3' gene-specific primers (3' GSP) according to your experimental workflow and target sequence information. Whether the goal is to extend toward the 5' end, amplify the 3' end, or validate transcript structure, the primer design can be customized to match your research needs.

Core Advantages

1. Supports Both 5' GSP and 3' GSP Design

Primer design can be performed for different RACE experiment directions.

Supported options include:

• 5' GSP design for 5' end amplification
• 3' GSP design for 3' end amplification
• Support for both 5' RACE and 3' RACE workflows
• Custom design based on known internal gene sequences

This allows flexible application in transcript end identification and full-length cDNA analysis.

2. Dedicated Design Software for Accurate and Reliable Results

Our professional team has developed specialized primer design software for RACE applications.

The design process evaluates key primer features, including:

• Tm value
• GC content
• Primer length
• Amplification direction
• Target sequence position
• Secondary structure risk
• Primer specificity

This helps improve amplification success rate and experimental reliability.

3. Customized Design Based on Experimental Goals

Each project can be tailored according to the customer’s target gene, available sequence information, and experimental purpose.

Customization may include:

• Primer position selection
• Nested primer design
• Outer and inner GSP design
• Adjustment of amplification region
• Optimization for difficult templates
• Design for gene cloning or transcript validation

This makes the service suitable for different RACE kits, protocols, and downstream applications.

4. No Limitation on Gene Length

The service can handle both short and long gene sequences.

Supported sequence types include:

• Short gene fragments
• Long transcripts
• Large coding sequences
• Complex gene sequences
• Long input FASTA sequences

Long sequences can be processed efficiently, enabling stable primer design even for challenging genes.

5. Suitable for Multiple Research Applications

The RACE Primer Design Service can be used in a wide range of molecular biology studies.

Typical applications include:

• Full-length cDNA cloning
• Unknown 5' or 3' end amplification
• Transcript boundary validation
• Alternative transcript analysis
• Gene structure annotation
• Functional gene research
• Expression and regulation studies

Service Deliverables

Each project can provide:

• 5' GSP or 3' GSP primer sequences
• Optional nested GSP primer design
• Primer position information
• Basic primer parameters
• Customized design notes based on the target sequence and experiment type