MSP/BSP Methylation Primer Design Service

Product Introduction
Our MSP/BSP Methylation Primer Design Service provides professional primer design solutions for DNA methylation analysis. The service is suitable for methylation-specific PCR, bisulfite sequencing PCR, epigenetic research, gene regulation studies, biomarker screening, and disease-related methylation detection.

By analyzing CpG-rich regions and bisulfite-converted sequence characteristics, we design ready-to-use primer sets for both methylated and unmethylated DNA templates, helping improve assay specificity, usability, and experimental efficiency.

Core Advantages

1. Automatic CpG Island Recognition and Accurate Methylation Hotspot Targeting

The system can intelligently analyze input sequences or gene names to identify CpG-rich regions and potential methylation hotspots.

Supported analysis features include:

• Automatic recognition of CpG islands
• Localization of CpG-rich target regions
• Identification of methylation-related hotspots
• Support for multiple species, including human, mouse, pig, bacteria, and other organisms

This helps researchers quickly locate suitable target regions for methylation analysis without extensive manual sequence screening.

2. One-Click Generation of Methylated and Unmethylated Primer Sets

The system designs primers separately based on the sequence characteristics after bisulfite conversion.

Design logic includes:

• Methylated template design: cytosine remains as C
• Unmethylated template design: cytosine is converted to T
• M primers for methylation-specific amplification
• U primers for unmethylated-template amplification

Each delivery includes two complete primer pairs:

• MF/MR: methylated forward and reverse primers
• UF/UR: unmethylated forward and reverse primers

Key benefits:

• Ready for direct PCR amplification
• No secondary sequence processing required
• Clear distinction between methylated and unmethylated templates
• Improved convenience for MSP/BSP experimental workflows